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Low Density SNP Marker Detection: KASP Typing
99.8
%
Accuracy rate exceeds
KASP technology, Kompetitive Allele Specific PCR,Through the design of specific primers and fluorescent probes, allele-specific amplification and detection of target SNP sites can be achieved.
It can accurately genotype a wide range of genomic DNA samples with an accuracy rate exceeding 99.8%.
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Principle of KASP
KASP marker
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Forward primer 1
Forward primer 2
Reverse universal primer
Universal fluorescent labeled primers
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Reporting group and quenching group labeled primer binding, no fluorescence emitted
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PCR first round: specific primer amplification
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Forward primer 1
Forward primer 2
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PCR second round: reverse universal primer amplification
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PCR third round: fluorescence generation
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After denaturation, the fluorescently labeled primers separate from the quencher-labeled primers and participate in the PCR reaction to produce fluorescence.
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Service Process
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